Ptychography by using pinhole illumination in lens-less microscopy

Iqra Bashir; Ahsan Mehmood Khan; Aqiba Hafeez; Anwar Hussain

doi:10.1117/12.2615838

7 December 2021 Ptychography by using pinhole illumination in lens-less microscopy

Iqra Bashir, Ahsan Mehmood Khan, Aqiba Hafeez, Anwar Hussain

Proceedings Volume 11922, Advances in Microscopic Imaging III; 119221O (2021) https://doi.org/10.1117/12.2615838
Event: European Conferences on Biomedical Optics, 2021, Online Only

Abstract

Ptychographic imaging is centered on the phase retrieval of the sample from the diffraction patterns recorded at the sensor. Ptychography is a lens less computational imaging method that has various types. The single aperture ptychographic imaging technique is discussed in this design. Firstly, the simulation is done by MATLAB and after that the physical experiment is performed. The amplitude and phase-reconstructed images are retrieved by pinhole illumination. The sample is illuminated through the pinhole (generated by SLM) and the encoded sample information propagates to the CMOS, which is positioned at an optimum distance and captures the images. The Ten different diffraction patterns of the specimen are recorded by the CMOS sensor by shifting it axially and the data is recorded on the computer. To retrieve the phase and amplitude information about sample from raw images the reconstruction process is applied through MATLAB. The recorded field is back propagated to the object plane in the reconstruction process. Finally, by including all the back propagated images, a final reconstructed image with higher resolution is obtained using the stochastic gradient descent technique. The noise suppression approach is added to the final image to further increase resolution. The final image is highly resolved. Lens-less microscopy generates a cost-effective, portable optical device. Ptychography have many applications in medical field. It is used within the human body to detect biological cells. This provides good results in diagnosis of diseases.

Citation Download Citation

Iqra Bashir, Ahsan Mehmood Khan, Aqiba Hafeez, and Anwar Hussain "Ptychography by using pinhole illumination in lens-less microscopy", Proc. SPIE 11922, Advances in Microscopic Imaging III, 119221O (7 December 2021); https://doi.org/10.1117/12.2615838

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
1 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Microscopy

Diffraction

Image resolution

MATLAB

Physics

Quantum optics

Roads

Show All Keywords

Keywords/Phrases

Search In:

Publication Years