Segmenting quantitative phase images of neurons using a deep learning model trained on images generated from a neuronal growth model

Eddie M. Gil; Zachary Steelman; Anna Sedelnikova; Joel N. Bixler

doi:10.1117/12.2608770

2 March 2022 Segmenting quantitative phase images of neurons using a deep learning model trained on images generated from a neuronal growth model

Eddie M. Gil, Zachary Steelman, Anna Sedelnikova, Joel N. Bixler

Author Affiliations +

Proceedings Volume 11970, Quantitative Phase Imaging VIII; 1197007 (2022) https://doi.org/10.1117/12.2608770
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Morphological changes in neurons are closely related to neurological disorders. Quantitative Phase Imaging (QPI) has been used to assess neuronal changes over time, using mass-sensitive contrast to quantitatively track network growth. QPI requires high quality segmentation of neurons in order to measure neuron cell body and neurite mass distributions. Neural networks are the state of the art for segmentation, but require thousands of images in order to generalize well. However, recent work on network functionality has shown that networks generalize by learning simple functions. Whether low data complexity hinders this has yet to be seen. Here we test this by simulating low complexity data, specifically, QPI images of neurons simulated using a neuronal growth model. We show segmentation results for feeding the network lab-acquired data.

Conference Presentation

Citation Download Citation

Eddie M. Gil, Zachary Steelman, Anna Sedelnikova, and Joel N. Bixler "Segmenting quantitative phase images of neurons using a deep learning model trained on images generated from a neuronal growth model", Proc. SPIE 11970, Quantitative Phase Imaging VIII, 1197007 (2 March 2022); https://doi.org/10.1117/12.2608770

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