An adaptive excitation source for multiphoton deep and fast imaging

Chris Xu

doi:10.1117/12.2609150

2 March 2022 An adaptive excitation source for multiphoton deep and fast imaging

Chris Xu

Proceedings Volume 11971, High-Speed Biomedical Imaging and Spectroscopy VII; 1197102 (2022) https://doi.org/10.1117/12.2609150
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Multiphoton microscopy (MPM) is the go-to technique for high spatial resolution, deep imaging in scattering biological tissue. The performance of MPM, e.g., imaging speed and volume, depends on the characteristics of the excitation source and is fundamentally limited by the signal photon budget. We developed an adaptive excitation source (AES). By feeding the structural information of the sample to the source, the AES produces on-demand pulses only in the regions of interest (ROIs). The AES therefore transforms a conventional multiphoton microscope into a “random-access” microscope that only excites the ROIs and performs the most photon efficient excitation for functional imaging.

Conference Presentation

Citation Download Citation

Chris Xu "An adaptive excitation source for multiphoton deep and fast imaging", Proc. SPIE 11971, High-Speed Biomedical Imaging and Spectroscopy VII, 1197102 (2 March 2022); https://doi.org/10.1117/12.2609150

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