Bio-organism detection using microsphere resonators in fluidics

B. J. Demory; L. T. Echeveria; S. Gilmore; C. Tolfa; S. Harrison; G. Chavez; P. K. Singh; A. S. P. Chang; T. Bond

doi:10.1117/12.2610234

4 March 2022 Bio-organism detection using microsphere resonators in fluidics

B. J. Demory, L. T. Echeveria, S. Gilmore, C. Tolfa, S. Harrison, G. Chavez, P. K. Singh, A. S. P. Chang, T. Bond

Proceedings Volume 11987, Laser Resonators, Microresonators, and Beam Control XXIV; 1198708 (2022) https://doi.org/10.1117/12.2610234
Event: SPIE LASE, 2022, San Francisco, California, United States

Abstract

The evolution of pathogens has increased the demand for a sensing and detection platform, capable of qualifying constituents in real time. Whispering Gallery Mode Resonators provide an ideal biochemical sensing platform due to their low cost, high sensitivity, and low impact on the analyte. These resonators have high quality factors and possess the ability to detect minute changes in the local environment, as the light traveling on the surface of the resonator, when at resonance interacts with the surrounding medium for interaction lengths on the order of ~10-100cm’s . These changes in physical properties are captured through shifts of the resonance wavelength, resonance dip intensity, and/or quality factor. In this work, we provide our design of a 3-D printed microfluidic cell that is compatible with our taper and sphere coupling scheme developed from our previous work. Initially, the baseline performance of the resonator fluidic system was established by measuring the resonance wavelength shift due to refractive index change from water to phosphate buffered saline (PBS). Next, we showcase our biofunctionalization procedure and measure the accumulation of pathogens, such as E. Coli and Influenza A, on the resonator’s surface. The presence of these biological analytes results in small changes in the resonator’s diameter and refractive index, which manifests in real time as a red shift of the resonance wavelength on the picometer scale. Finally, we develop the foundation for a silicon integrated circuit chip resonator system, resulting in a further reduction of our system’s footprint.

Conference Presentation

Citation Download Citation

B. J. Demory, L. T. Echeveria, S. Gilmore, C. Tolfa, S. Harrison, G. Chavez, P. K. Singh, A. S. P. Chang, and T. Bond "Bio-organism detection using microsphere resonators in fluidics", Proc. SPIE 11987, Laser Resonators, Microresonators, and Beam Control XXIV, 1198708 (4 March 2022); https://doi.org/10.1117/12.2610234

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