Picosecond fluorescence microscopy

Motoyuki Watanabe; Musubu Koishi; Hirofumi Kan; Kenneth J. Kaufmann; Yutaka Tsuchiya

doi:10.1117/12.17901

1 April 1990 Picosecond fluorescence microscopy

Motoyuki Watanabe, Musubu Koishi, Hirofumi Kan, Kenneth J. Kaufmann, Yutaka Tsuchiya

Proceedings Volume 1209, Picosecond and Femtosecond Spectroscopy from Laboratory to Real World; (1990) https://doi.org/10.1117/12.17901
Event: OE/LASE '90, 1990, Los Angeles, CA, United States

Abstract

A new picosecond time-resolved fluorescence microscope (PFM) has been developed for measuring and mapping the lifetime of laser excited fluorescence from a sample placed under a micro— scope. A compact laser diode is used to generate excitation pulses. The combination of a spectrograph and a streak camera performs time resolved fluorescence spectroscopy with a resolution of 40 ps ( 5 ps with deconvolusion). The lifetime and the intensity of laser excited fluorescence are analyzed b a computer and are displayed on a monitor as either a lifetime or intensity map.

Citation Download Citation

Motoyuki Watanabe, Musubu Koishi, Hirofumi Kan, Kenneth J. Kaufmann, and Yutaka Tsuchiya "Picosecond fluorescence microscopy", Proc. SPIE 1209, Picosecond and Femtosecond Spectroscopy from Laboratory to Real World, (1 April 1990); https://doi.org/10.1117/12.17901

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