PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
There is a need for time efficient evaluation methods to discriminate between viable and dead bacterial spores. In this work, the potential to use the autofluorescence from spore suspensions for evaluation of spore deactivation processes is investigated. Bacillus thuringiensis and Bacillus anthracis ATCC 4229 spores were exposed to UV-radiation for deactivation and the fluorescence response was monitored at different radiation doses and the deactivation was evaluated via traditional bacterial incubation on agar culture plates. For excitation wavelengths of, e.g., 280 m and 330 nm, differences in the fluorescence response could be observed for different live:dead ratios.
Alexandra C. Johansson,Lars Landström,Rasmus Öberg, andPer Ola Andersson
"Monitoring deactivation processes of bacterial spores using fluorescence spectroscopy", Proc. SPIE 12116, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XXIII, 121160M (30 May 2022); https://doi.org/10.1117/12.2623447
ACCESS THE FULL ARTICLE
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
The alert did not successfully save. Please try again later.
Alexandra C. Johansson, Lars Landström, Rasmus Öberg, Per Ola Andersson, "Monitoring deactivation processes of bacterial spores using fluorescence spectroscopy," Proc. SPIE 12116, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XXIII, 121160M (30 May 2022); https://doi.org/10.1117/12.2623447