Monitoring deactivation processes of bacterial spores using fluorescence spectroscopy

Alexandra C. Johansson; Lars Landström; Rasmus Öberg; Per Ola Andersson

doi:10.1117/12.2623447

30 May 2022 Monitoring deactivation processes of bacterial spores using fluorescence spectroscopy

Alexandra C. Johansson, Lars Landström, Rasmus Öberg, Per Ola Andersson

Proceedings Volume 12116, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XXIII; 121160M (2022) https://doi.org/10.1117/12.2623447
Event: SPIE Defense + Commercial Sensing, 2022, Orlando, Florida, United States

Abstract

There is a need for time efficient evaluation methods to discriminate between viable and dead bacterial spores. In this work, the potential to use the autofluorescence from spore suspensions for evaluation of spore deactivation processes is investigated. Bacillus thuringiensis and Bacillus anthracis ATCC 4229 spores were exposed to UV-radiation for deactivation and the fluorescence response was monitored at different radiation doses and the deactivation was evaluated via traditional bacterial incubation on agar culture plates. For excitation wavelengths of, e.g., 280 m and 330 nm, differences in the fluorescence response could be observed for different live:dead ratios.

Conference Presentation

Citation Download Citation

Alexandra C. Johansson, Lars Landström, Rasmus Öberg, and Per Ola Andersson "Monitoring deactivation processes of bacterial spores using fluorescence spectroscopy", Proc. SPIE 12116, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XXIII, 121160M (30 May 2022); https://doi.org/10.1117/12.2623447

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