Digital holographic microscopy for label-free in vitro cytotoxicity testing of polymeric nanocarriers: an interlaboratory comparison

Anne Marzi; Kai Moritz Eder; Álvaro Barroso; Ane Marit Wågbø; Torkild Visnes; Ruth B. Schmid; Geir Klinkenberg; Björn Kemper; Jürgen Schnekenburger

doi:10.1117/12.2649808

16 March 2023 Digital holographic microscopy for label-free in vitro cytotoxicity testing of polymeric nanocarriers: an interlaboratory comparison

Anne Marzi, Kai Moritz Eder, Álvaro Barroso, Ane Marit Wågbø, Torkild Visnes, Ruth B. Schmid, Geir Klinkenberg, Björn Kemper, Jürgen Schnekenburger

Author Affiliations +

Proceedings Volume 12389, Quantitative Phase Imaging IX; 123890F (2023) https://doi.org/10.1117/12.2649808
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Digital holographic microscopy (DHM) has been demonstrated to be a suitable label-free and non-invasive quantitative phase imaging (QPI) tool in risk assessment of the cytotoxic potential of engineered nanoparticles and organic nanocarriers. For a broader application, robustness of DHM-based assays needs to be demonstrated towards DHM standardization in risk assessment. Thus, we performed an interlaboratory comparison on the transferability and reproducibility of a DHM-based assay. The cytotoxic potential of organic nanoparticles on A549 lung epithelial cells was analyzed in two European laboratories using identically constructed DHM systems. Our results demonstrate a solid and accurate performance of the DHM-based cytotoxicity assay

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Anne Marzi, Kai Moritz Eder, Álvaro Barroso, Ane Marit Wågbø, Torkild Visnes, Ruth B. Schmid, Geir Klinkenberg, Björn Kemper, and Jürgen Schnekenburger "Digital holographic microscopy for label-free in vitro cytotoxicity testing of polymeric nanocarriers: an interlaboratory comparison", Proc. SPIE 12389, Quantitative Phase Imaging IX, 123890F (16 March 2023); https://doi.org/10.1117/12.2649808

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