Fluorescence and Raman imaging of amyloid plaques reveals carotenoid accumulations

Benjamin Lochocki; Loes Ettema; Jeroen J. M. Hoozemans; Johannes F. de Boer; Freek Ariese

doi:10.1117/12.2670851

11 August 2023 Fluorescence and Raman imaging of amyloid plaques reveals carotenoid accumulations

Benjamin Lochocki, Loes Ettema, Jeroen J. M. Hoozemans, Johannes F. de Boer, Freek Ariese

Proceedings Volume 12627, Translational Biophotonics: Diagnostics and Therapeutics III; 126270C (2023) https://doi.org/10.1117/12.2670851
Event: European Conferences on Biomedical Optics, 2023, Munich, Germany

Abstract

Alzheimer’s disease (AD) is characterized by the presence of extracellular deposits of amyloid-beta peptides (known as AD plaques). Its assessment is usually achieved post-mortem, requiring chemical pre-treatment via an antibody or indirect labelling. Label-free imaging techniques, like auto-fluorescence, spontaneous Raman (SpR) and stimulated Raman (SRS) imaging could be performed on tissue in its native state to study the biomolecular composition of AD plaques and contribute to a better understanding of the disease. Here we present imaging results of human brain amyloid core plaques. We show blue and green autofluorescence emission localized at the same plaque position while Raman spectroscopy revealed the presence of carotenoids at the same spot. For identifying the underlying carotenoids, first carotenoid reference spectra in hexane solution and then adsorbed on aggregated Aβ42 peptides were recorded. From the six carotenoids measured, lycopene matched closest with the Raman peak positions observed in the measured AD plaque. Furthermore, we used SRS to investigate the presence of a lipid halo around plaque locations as reported in literature for transgenic AD mice.

Citation Download Citation

Benjamin Lochocki, Loes Ettema, Jeroen J. M. Hoozemans, Johannes F. de Boer, and Freek Ariese "Fluorescence and Raman imaging of amyloid plaques reveals carotenoid accumulations", Proc. SPIE 12627, Translational Biophotonics: Diagnostics and Therapeutics III, 126270C (11 August 2023); https://doi.org/10.1117/12.2670851

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
3 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Raman spectroscopy

Fluorescence

Fluorescence imaging

Auto-fluorescence imaging

Body composition

Brain

Brain diseases

Show All Keywords

Keywords/Phrases

Search In:

Publication Years