Digital inline holographic microscopy for fast, label-free detection of antimicrobial mechanism of action

Zohreh Sedaghat; Héloïse Botrel; Alexei Novoloaca; Mélanie Nehlich; Christophe Védrine; Sophie Dixneuf

doi:10.1117/12.2670536

11 August 2023 Digital inline holographic microscopy for fast, label-free detection of antimicrobial mechanism of action

Zohreh Sedaghat, Héloïse Botrel, Alexei Novoloaca, Mélanie Nehlich, Christophe Védrine, Sophie Dixneuf

Author Affiliations +

Proceedings Volume 12629, Emerging Technologies for Cell and Tissue Characterization II; 126290Q (2023) https://doi.org/10.1117/12.2670536
Event: European Conferences on Biomedical Optics, 2023, Munich, Germany

Abstract

This work is a contribution to the current effort in developing rapid, robust and cost-effective technologies for antimicrobial classification according to their mechanism of action, which could potentially serve as a new MoA detector. We propose an original, label-free technology based on time-lapse Digital Inline Holographic Microscopy, which will be coupled to deep-learning analysis in a later stage. This paper focuses on the physical analysis of the reconstructed time-lapse phase image at the single-cell level, and shows that time-lapse DIHM is able to capture different phenotypes linked to different antibiotic classes. PCA analysis based on 10 antibiotics for 4 inhibiting targets shows that we can discriminate 5 over 6 chemical classes in less than 2 hours.

Citation Download Citation

Zohreh Sedaghat, Héloïse Botrel, Alexei Novoloaca, Mélanie Nehlich, Christophe Védrine, and Sophie Dixneuf "Digital inline holographic microscopy for fast, label-free detection of antimicrobial mechanism of action", Proc. SPIE 12629, Emerging Technologies for Cell and Tissue Characterization II, 126290Q (11 August 2023); https://doi.org/10.1117/12.2670536

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