Mueller matrix polarimetric imaging of whole-slide tissue samples

Ariel Fernández; Roman Demczylo; Diego Silva

doi:10.1117/12.2669789

9 August 2023 Mueller matrix polarimetric imaging of whole-slide tissue samples

Ariel Fernández, Roman Demczylo, Diego Silva

Proceedings Volume 12630, Advances in Microscopic Imaging IV; 1263014 (2023) https://doi.org/10.1117/12.2669789
Event: European Conferences on Biomedical Optics, 2023, Munich, Germany

Abstract

Interaction of polarized light with tissue samples is an emergent and powerful tool for biomedical diagnosis due to the possibility of providing quantitative information through Stokes parameters and Mueller matrix elements. Division of Focal Plane polarimetric sensing in particular has recently become popular due to advances in technology and commercial availability of microgrid polarizers integrated onto the sensing chip of a camera. After calibration of the system, intensity measures from these sensors can be mapped to the State of Polarization (SoP) of the input. A microscopy setup incorporating DoFP sensing can allow then to obtain Stokes parameters corresponding to a given Field of View (FoV) of a tissue sample. By illuminating with linearly independent SoP in the input, Mueller matrix elements can also be retrieved for the FoV of the sample. Finally, FoV extension to whole-slide Mueller matrix imaging can be achieved through stitching of multiple images taken by means of a motorized XY stage. Validation results for whole-slide Mueller matrix elements of tissue samples are presented.

Citation Download Citation

Ariel Fernández, Roman Demczylo, and Diego Silva "Mueller matrix polarimetric imaging of whole-slide tissue samples", Proc. SPIE 12630, Advances in Microscopic Imaging IV, 1263014 (9 August 2023); https://doi.org/10.1117/12.2669789

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