A compact, turn-key platform for multiplex stimulated Raman scattering microscopy

Francesco Crisafi; Benedetta Talone; Andrea Ragni; Gabriele Di Noia; Mujeeb Rahman; Jing He; Jeremiah Marcellino; Goutam Kar; Yarjan Samad; Boyang Mao; Renzo Vanna; Franziska Hoffmann; Orlando Guntinas-Lichius; Sze Yun Set; Andrea C. Ferrari; Giulio Cerullo; Matteo Negro

doi:10.1117/12.3002592

12 March 2024 A compact, turn-key platform for multiplex stimulated Raman scattering microscopy

Francesco Crisafi, Benedetta Talone, Andrea Ragni, Gabriele Di Noia, Mujeeb Rahman, Jing He, Jeremiah Marcellino, Goutam Kar, Yarjan Samad, Boyang Mao, Renzo Vanna, Franziska Hoffmann, Orlando Guntinas-Lichius, Sze Yun Set, Andrea C. Ferrari, Giulio Cerullo, Matteo Negro

Author Affiliations +

Proceedings Volume 12839, Biomedical Vibrational Spectroscopy 2024: Advances in Research and Industry; 1283904 (2024) https://doi.org/10.1117/12.3002592
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

We combine an all-fiber dual wavelength, self-synchronized laser and a dedicated multi-channel detection unit to perform state-of-the-art multiplex Stimulated Raman Scattering (SRS) microscopy. The system covers the full CH spectrum in 1 μs reaching shot-noise limited performances with 25 μW per detection channel. This all-inone solution is based on a passively synchronized dual-wavelength laser source with shot-noise limited relative intensity noise from 600 kHz and a modular multi-channel lock-in detection unit. The synergistic design between laser source and detection system simplifies multiplex SRS implementation for real-time full-chemical imaging.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Francesco Crisafi, Benedetta Talone, Andrea Ragni, Gabriele Di Noia, Mujeeb Rahman, Jing He, Jeremiah Marcellino, Goutam Kar, Yarjan Samad, Boyang Mao, Renzo Vanna, Franziska Hoffmann, Orlando Guntinas-Lichius, Sze Yun Set, Andrea C. Ferrari, Giulio Cerullo, and Matteo Negro "A compact, turn-key platform for multiplex stimulated Raman scattering microscopy", Proc. SPIE 12839, Biomedical Vibrational Spectroscopy 2024: Advances in Research and Industry, 1283904 (12 March 2024); https://doi.org/10.1117/12.3002592

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available