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An epifluorescence microscope setup has been developed and tested featuring Raman and multiplex-CARS capabilities. Samples were stored in 96 multi well plates on a sample stage with triaxial piezo drive stages for sample scanning. Raman excitation was achieved by a 785 nm laser with up to 100 mW output power and below 1 pm wavelength bandwidth (FWHM). Multiplex CARS was excited by a laser system providing 1064 nm primary pulses with 1 ns duration and broadband “white” anti-Stokes radiation (1100 nm … 1700 nm) generated by a non-linear optical fiber. Repetition rates around 30 kHz resulted in an average excitation power of up to 200 mW. For both excitation principles the signal was captured by a Raman spectrograph (Ocean Insight QEPRO) designed for 790 nm … 1070 nm spectral range, resolution below 1 nm and a thermoelectrically cooled detector for a good signal-to-noise ratio (SNR) to detect weak RAMAN and CARS signals. First measurements have been performed on selected samples and promising results have been achieved.
Heinrich Grüger,Jens Knobbe, andTim Schulze
"Design of an epi fluorescence microscope with multiplex CARS for marker free analysis", Proc. SPIE 12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024, 128540T (12 March 2024); https://doi.org/10.1117/12.3002136
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Heinrich Grüger, Jens Knobbe, Tim Schulze, "Design of an epi fluorescence microscope with multiplex CARS for marker free analysis," Proc. SPIE 12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024, 128540T (12 March 2024); https://doi.org/10.1117/12.3002136