Establishing rapid and efficient plant regeneration for poplar 741 through cluster seedling differentiation

Yunjiao Xu; Yurong Sun; Xue Wang; Baojun Zhang

doi:10.1117/12.3044468

27 August 2024 Establishing rapid and efficient plant regeneration for poplar 741 through cluster seedling differentiation

Yunjiao Xu, Yurong Sun, Xue Wang, Baojun Zhang

Author Affiliations +

Proceedings Volume 13252, Fourth International Conference on Biomedicine and Bioinformatics Engineering (ICBBE 2024); 1325210 (2024) https://doi.org/10.1117/12.3044468
Event: 2024 Fourth International Conference on Biomedicine and Bioinformatics Engineering (ICBBE 2024), 2024, Kaifeng, China

Abstract

Improving the regeneration efficiency of poplar trees is an important role in their genetic transformation. In this study, a regeneration system of 741 poplar tree, a hybrid descendant of Populus alba, was developed through cluster seedling differentiation. By utilizing different hormone concentrations and their combinations to induce callus tissue, cluster seedling formation, and cluster seedling stem extraction, the regeneration system of 741 poplar planting plants was optimized. The optimized concentration and combination of induced callus culture hormones is 1.0 mg/L 6-BA+0.2 mg/L NAA, that of cluster seedlings differentiation is 0.5 mg/L 6-BA+0.1 mg/L NAA, and that of stem extraction is 0.3 mg/L 6-BA+0.05 mg/L NAA. In addition, compared with normal photoperiod, it was found that continuous dark cultivation of tender leaves for 7 days and of petioles for 5 days significantly increased the induction rate of callus tissue. In summary, we established an efficient regeneration system for 741 poplar via cluster seedling differentiation involved in hormone level and combination optimization, photoperiod regulation, and sucrose concentration screening, which provides assurance for genetic transformation of poplar.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Yunjiao Xu, Yurong Sun, Xue Wang, and Baojun Zhang "Establishing rapid and efficient plant regeneration for poplar 741 through cluster seedling differentiation", Proc. SPIE 13252, Fourth International Conference on Biomedicine and Bioinformatics Engineering (ICBBE 2024), 1325210 (27 August 2024); https://doi.org/10.1117/12.3044468

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KEYWORDS

Tissues

Genetics

Cultural studies

Astatine

Statistical analysis

Explants

Relative humidity

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