Use of fluorescence spectroscopy to elucidate structural features of the nicotinic acetylcholine receptor

David Allen Johnson; C. Fernando Valenzuela

doi:10.1117/12.44210

1 June 1991 Use of fluorescence spectroscopy to elucidate structural features of the nicotinic acetylcholine receptor

David Allen Johnson, C. Fernando Valenzuela

Proceedings Volume 1432, Biomolecular Spectroscopy II; (1991) https://doi.org/10.1117/12.44210
Event: Optics, Electro-Optics, and Laser Applications in Science and Engineering, 1991, Los Angeles, CA, United States

Abstract

The authors review three examples of how fluorescence spectroscopic techniques have been employed to elucidate structural features of the nicotinic acetylcholine receptor from the electric organ of Torpedo californica. The instrumentation and most relevant fluorescence methods utilized for these studies are briefly discussed. The examples reviewed are as follows: (1) the measurement of the average distance between the two acetylcholine binding sites on the receptor and the noncompetitive inhibitor binding site in the central ion channel, (2) the measurement of distance between fluorescently labeled snake (alpha) -toxins bound to the same membrane-associated receptor oligomer and bound to the adjacent receptor oligomers, and (3) the relative orientation of snake (alpha) -toxin with respect to the central ion channel and the plane of the lipid membrane into which the receptor is embedded.

Citation Download Citation

David Allen Johnson and C. Fernando Valenzuela "Use of fluorescence spectroscopy to elucidate structural features of the nicotinic acetylcholine receptor", Proc. SPIE 1432, Biomolecular Spectroscopy II, (1 June 1991); https://doi.org/10.1117/12.44210

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