Digital imaging microscopy: the marriage of spectroscopy and the solid state CCD camera

Thomas M. Jovin; Donna J. Arndt-Jovin

doi:10.1117/12.50457

1 December 1991 Digital imaging microscopy: the marriage of spectroscopy and the solid state CCD camera

Proceedings Volume 1439, International Conference on Scientific Optical Imaging; (1991) https://doi.org/10.1117/12.50457
Event: International Conference on Scientific Optical Imaging, 1990, Georgetown, Cayman Islands

Abstract

Biological samples have been imaged using microscopes equipped with slow-scan CCD cameras. Examples are presented of studies based on the detection of light emission signals in the form of fluorescence and phosphorescence. They include applications in the field of cell biology: (a) replication and topology of mammalian cell nuclei; (b) cytogenetic analysis of human metaphase chromosomes; and (c) time-resolved measurements of DNA-binding dyes in cells and on isolated chromosomes, as well as of mammalian cell surface antigens, using the phosphorescence of acridine orange and fluorescence resonance energy transfer of labeled lectins, respectively.

Citation Download Citation

Thomas M. Jovin and Donna J. Arndt-Jovin "Digital imaging microscopy: the marriage of spectroscopy and the solid state CCD camera", Proc. SPIE 1439, International Conference on Scientific Optical Imaging, (1 December 1991); https://doi.org/10.1117/12.50457

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