Imaging DNA molecules chemically bound to a mica surface

Stuart M. Lindsay; Yuri L. Lyubchenko; Anna S. Gall; L. S. Shlyakhtenko; R. E. Harrington

doi:10.1117/12.58177

1 May 1992 Imaging DNA molecules chemically bound to a mica surface

Stuart M. Lindsay, Yuri L. Lyubchenko, Anna S. Gall, L. S. Shlyakhtenko, R. E. Harrington

Proceedings Volume 1639, Scanning Probe Microscopies; (1992) https://doi.org/10.1117/12.58177
Event: OE/LASE '92, 1992, Los Angeles, CA, United States

Abstract

We describe a procedure for imaging DNA molecules using atomic force microscopy (AFM). Double-stranded DNA binds to a mica surface modified by treatment with 3- aminopropyltriethoxysilane to yield AFM images that are stable under repeated scanning. We used this procedure to obtain high quality images of unstained linear DNA molecules and circular DNA molecules. The resolution was limited to tens of nanometers by the profile of the AFM tips.

Citation Download Citation

Stuart M. Lindsay, Yuri L. Lyubchenko, Anna S. Gall, L. S. Shlyakhtenko, and R. E. Harrington "Imaging DNA molecules chemically bound to a mica surface", Proc. SPIE 1639, Scanning Probe Microscopies, (1 May 1992); https://doi.org/10.1117/12.58177

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available