Novel dual-channel time-domain phosphorimeter for time-resolved spectroscopic studies of biomolecules

Liqun Yang; Daniel McStay; Abhay Sharma; Alan J. Rogers; Peter J. Quinn

doi:10.1117/12.58244

1 April 1992 Novel dual-channel time-domain phosphorimeter for time-resolved spectroscopic studies of biomolecules

Liqun Yang, Daniel McStay, Abhay Sharma, Alan J. Rogers, Peter J. Quinn

Proceedings Volume 1640, Time-Resolved Laser Spectroscopy in Biochemistry III; (1992) https://doi.org/10.1117/12.58244
Event: OE/LASE '92, 1992, Los Angeles, CA, United States

Abstract

A newly developed dual-channel time domain phosphorimeter is described in this paper. This employs a pulsed, frequency doubled Nd:YAG laser as a linear polarized pumping source. The resulting orthogonally polarized emission components are then simultaneously collected by two oppositely positioned photomultiplier tubes (PMTs), digitized by a digital storage adaptor, and then transferred to an IBM PC microcomputer where the intensity or the depolarization (anisotropy) of the emission is calculated. The range of lifetimes or rotational correlation times that can be routinely determined with this instrument extends from a few microseconds to milliseconds. The instrument has an 8 bit magnitude resolution and a signal sampling rate of 20 mesa-samples per second. The averaging function of the instrument yields a single measurement with an average of over 2 to 255 excitation transients. To demonstrate the utility of the new instrument, studies of the emission properties of the triplet probe eosin immobilized in a polymer matrix and covalently bound to a membrane protein are reported.

Citation Download Citation

Liqun Yang, Daniel McStay, Abhay Sharma, Alan J. Rogers, and Peter J. Quinn "Novel dual-channel time-domain phosphorimeter for time-resolved spectroscopic studies of biomolecules", Proc. SPIE 1640, Time-Resolved Laser Spectroscopy in Biochemistry III, (1 April 1992); https://doi.org/10.1117/12.58244

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