Promotion of porphyrin cytotoxicity by ultrasound (Invited Paper)

David Kessel; Russell Jeffers; Charles A. Cain

doi:10.1117/12.60930

1 June 1992 Promotion of porphyrin cytotoxicity by ultrasound (Invited Paper)

David Kessel, Russell Jeffers, Charles A. Cain

Proceedings Volume 1645, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy; (1992) https://doi.org/10.1117/12.60930
Event: OE/LASE '92, 1992, Los Angeles, CA, United States

Abstract

We examined the effect of ultrasound on the murine leukemia L1210 cell line in culture, in the presence of different photosensitizing agents: mesoporphyrin and tetraphenylporphines bearing 1 - 4 sulfonate groups. These agents could enhance ultrasound-induced cell damage when added to the incubation medium; sensitizers present only in the intracellular space were inactive in this regard. All of the porphine sulfonates were equally effective sensitizers to ultrasound but the product with 2 adjacent-SO₃H groups was the most effective photodynamic sensitizer. Ultrasound-induced cytotoxicity was associated with outer membrane damage and could be detected by measuring hydrophobic- or charge-associated whole-cell partitioning or by measurement of inhibition of active transport of a non-metabolized amino acid.

Citation Download Citation

David Kessel, Russell Jeffers, and Charles A. Cain "Promotion of porphyrin cytotoxicity by ultrasound (Invited Paper)", Proc. SPIE 1645, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy, (1 June 1992); https://doi.org/10.1117/12.60930

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