Autofluorescence distribution in skin tissue revealed by microspectrophotometer measurements

Haishan Zeng; Calum E. MacAulay; Branko Palcic; David I. McLean M.D.

doi:10.1117/12.147020

1 July 1993 Autofluorescence distribution in skin tissue revealed by microspectrophotometer measurements

Haishan Zeng, Calum E. MacAulay, Branko Palcic, David I. McLean M.D.

Proceedings Volume 1876, Lasers in Otolaryngology, Dermatology, and Tissue Welding; (1993) https://doi.org/10.1117/12.147020
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

The autofluorescence distribution of excised skin tissue sections were studied using a novel microspectrophotometer (MSP) system developed in our laboratory. The MSP consists of a microscope, an Optical Multichannel Analyzer (OMA), and a PC computer. Skin samples were placed on a glass slide for microscopic examination. A 442 nm He-Cd laser beam was used for fluorescence excitation and autofluorescence images were detected and recorded by a CCD camera. An optical fiber was mounted into the image plane of the microscope both to collect light from a specific micro-location in the sample for spectral analysis and to conduct light in the reverse direction into the microscope for alignment purposes. Examination of 24 human skin sections revealed that the dermis had a strong fluorescence emission, the stratum corneum had a weak fluorescence signal, while the remaining epidermis fluoresced with only a faint signal. The color of the fluorescence from the upper dermis and the lower dermis were different. Spectral analysis using the MSP showed that the positions of the autofluorescence spectra maxima from the upper and lower dermis differed by 20 nm suggesting that fundamentally different fluorophores may be present in the respective tissue layers. The border between the two different autofluorescence color areas was located approximately 480 micrometers below the dermal-epidermal junction. Whether this is the border between the papillary dermis and the reticular dermis has yet to be determined.

Citation Download Citation

Haishan Zeng, Calum E. MacAulay, Branko Palcic, and David I. McLean M.D. "Autofluorescence distribution in skin tissue revealed by microspectrophotometer measurements", Proc. SPIE 1876, Lasers in Otolaryngology, Dermatology, and Tissue Welding, (1 July 1993); https://doi.org/10.1117/12.147020

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