What light scattering can tell about the ordering of collagen fibrils in corneal stroma

Richard A. Farrell; David E. Freund; Russell L. McCally

doi:10.1117/12.148356

21 July 1993 What light scattering can tell about the ordering of collagen fibrils in corneal stroma

Richard A. Farrell, David E. Freund, Russell L. McCally

Proceedings Volume 1884, Static and Dynamic Light Scattering in Medicine and Biology; (1993) https://doi.org/10.1117/12.148356
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

Normal cornea transmits greater than 90% of visible light, but its transmission would be less than 30% if the stroma's collagen fibrils scattered independently of one another. Thus modern transparency theories are based on there being sufficient order in fibril positions for destructive interference to cause cancellation among the scattered fields. Two types of structure have been proposed: long-range crystalline order as used in the earliest theory, and short-range liquid-like order such as that depicted by electron microscopy. Of course structures depicted in electron micrographs may be distorted and other tests are required to determine the nature of the order. Light scattering measurements can afford such a test. Specifically, the two types of order produce different dependencies on wavelength for the scattering cross-section (angular or total) in the long-wavelength limit. Measurements must be analyzed appropriately to obtain the long-wavelength limit. The results reported in this paper demonstrate that measurements of both angular and total scattering cross-sections support short-range order of fibril positions.

Citation Download Citation

Richard A. Farrell, David E. Freund, and Russell L. McCally "What light scattering can tell about the ordering of collagen fibrils in corneal stroma", Proc. SPIE 1884, Static and Dynamic Light Scattering in Medicine and Biology, (21 July 1993); https://doi.org/10.1117/12.148356

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available