Study of pyruvate decarboxylase and thiamine kinase from brewer's yeast by SERS

Sergei A. Maskevich; Ivan P. Chernikevich; Gennedy A. Gachko; Leonid N. Kivach; Nataliya D. Strekal

doi:10.1117/12.146134

17 June 1993 Study of pyruvate decarboxylase and thiamine kinase from brewer's yeast by SERS

Sergei A. Maskevich, Ivan P. Chernikevich, Gennedy A. Gachko, Leonid N. Kivach, Nataliya D. Strekal

Proceedings Volume 1921, Laser Spectroscopy of Biomolecules; (1993) https://doi.org/10.1117/12.146134
Event: Laser Spectroscopy of Biomolecules: 4th International Conference on Laser Applications in Life Sciences, 1992, Jyvaskyla, Finland

Abstract

The Surface Enhanced Raman Scattering (SERS) spectra of holopyruvate decarboxylase (PDC) and thiamine kinase (ThK) adsorbed on silver electrode were obtained. In contrast to the Raman, the SERS spectrum of PDC contained no modes of tryptophan residues, it indicates a removal of this moiety from the surface. In the SERS spectrum of ThK the bands belonging to ligands bound to the protein were observed. A correlation between the SERS signal intensity and the enzymatic activity of the ThK separate fraction and found. The influence of amino acids on SERS spectra of thiamine (Th) was studied to determine the possible composition on microsurrounding of coenzyme.

Citation Download Citation

Sergei A. Maskevich, Ivan P. Chernikevich, Gennedy A. Gachko, Leonid N. Kivach, and Nataliya D. Strekal "Study of pyruvate decarboxylase and thiamine kinase from brewer's yeast by SERS", Proc. SPIE 1921, Laser Spectroscopy of Biomolecules, (17 June 1993); https://doi.org/10.1117/12.146134

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