Visible and UV Raman spectroscopy of membrane-bound peptides

Hideo Takeuchi; Teruhiko Maruyama; Yoshikazu Ohtsuka; Issei Harada

doi:10.1117/12.146140

17 June 1993 Visible and UV Raman spectroscopy of membrane-bound peptides

Hideo Takeuchi, Teruhiko Maruyama, Yoshikazu Ohtsuka, Issei Harada

Proceedings Volume 1921, Laser Spectroscopy of Biomolecules; (1993) https://doi.org/10.1117/12.146140
Event: Laser Spectroscopy of Biomolecules: 4th International Conference on Laser Applications in Life Sciences, 1992, Jyvaskyla, Finland

Abstract

Two Raman spectroscopic methods are presented to investigate the structures of membrane- bound peptides. In the first method, water accessibility to each tryptophan side chain of gramicidin A incorporated into phospholipid liposomes has been measured by use of hydrogen-deuterium exchange, selective isotopic labeling, and visible Raman spectroscopy. The water accessibility data are best explained by a newly proposed ion channel structure of gramicidin A. In the second method, we utilize ultraviolet resonance Raman intensity as a probe of environments of aromatic residues. Application of this method to enkephalin has shown that the tyrosine side chain is buried in the hydrophobic region of the lipid membrane while the phenylalanine side chain is not.

Citation Download Citation

Hideo Takeuchi, Teruhiko Maruyama, Yoshikazu Ohtsuka, and Issei Harada "Visible and UV Raman spectroscopy of membrane-bound peptides", Proc. SPIE 1921, Laser Spectroscopy of Biomolecules, (17 June 1993); https://doi.org/10.1117/12.146140

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