Novel L-glutamate assay based on fluorescence quenching

Ashutosh Sharma; Nigel S. M. Quantrill

doi:10.1117/12.180765

28 July 1994 Novel L-glutamate assay based on fluorescence quenching

Ashutosh Sharma, Nigel S. M. Quantrill

Proceedings Volume 2131, Biomedical Fiber Optic Instrumentation; (1994) https://doi.org/10.1117/12.180765
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

A novel optical procedure for measuring L-glutamate concentrations is described that exploits the quenching of thionine fluorescence by NADH, the latter being generated due to the reduction of NAD⁺ in an enzymatic reaction between L-glutamate dehydrogenase (GlDH) and L-glutamate. A substrate induced quenching (SIQ) constant of 660 (+/- 140) M^-1 at 200 s is obtained. The reported method was investigated over the L- glutamate concentration range of 0 - 800 (mu) M and offers a detection limit of 6 (mu) M.

Citation Download Citation

Ashutosh Sharma and Nigel S. M. Quantrill "Novel L-glutamate assay based on fluorescence quenching", Proc. SPIE 2131, Biomedical Fiber Optic Instrumentation, (28 July 1994); https://doi.org/10.1117/12.180765

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