Protein and cell fourier transform infrared-attenuated total internal reflectance on silver halide fibers under reagent-accessible conditions

Edward M. Kosower; Orit Marom-Albeck; Idan Pais; Abraham Katzir; Nechama S. Kosower

doi:10.1117/12.180767

28 July 1994 Protein and cell fourier transform infrared-attenuated total internal reflectance on silver halide fibers under reagent-accessible conditions

Edward M. Kosower, Orit Marom-Albeck, Idan Pais, Abraham Katzir, Nechama S. Kosower

Author Affiliations +

Proceedings Volume 2131, Biomedical Fiber Optic Instrumentation; (1994) https://doi.org/10.1117/12.180767
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

We describe a Fourier transform infrared-attenuated total internal reflectance (FTIR-ATR) technique for samples of 10 - 30 (mu) g deposited on a polycrystalline silver halide fiber. A protein deposit consists of approximately two molecular layers, for which the kinetics and extent of exchange with D₂O/N₂ are readily measured. We have extended the technique in several ways. Formation of complexes with modest association constants by taking advantage of the combination of protein mobility on the fiber with the absence of water. Association is thus favored, and the rates and extent of deuterium-hydrogen exchange may be followed as well as conformational changes. Polarized spectra of red blood cell ghosts have been measured on a flattened silver halide fiber. The cells containing the silver halide fibers have a design that allows controllable access to the sample. In addition to requiring only small quantities for good spectra, the cell is readily cleaned and handled. We anticipate the application of these techniques to many other biological samples, including mutant proteins.

Citation Download Citation

Edward M. Kosower, Orit Marom-Albeck, Idan Pais, Abraham Katzir, and Nechama S. Kosower "Protein and cell fourier transform infrared-attenuated total internal reflectance on silver halide fibers under reagent-accessible conditions", Proc. SPIE 2131, Biomedical Fiber Optic Instrumentation, (28 July 1994); https://doi.org/10.1117/12.180767

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