Multidisciplinary approach for detecting subcellular objects in low level light fluorescence microscopy

Amir Waks; Hershel Ginsburg

doi:10.1117/12.175171

5 May 1994 Multidisciplinary approach for detecting subcellular objects in low level light fluorescence microscopy

Amir Waks, Hershel Ginsburg

Proceedings Volume 2173, Image Acquisition and Scientific Imaging Systems; (1994) https://doi.org/10.1117/12.175171
Event: IS&T/SPIE 1994 International Symposium on Electronic Imaging: Science and Technology, 1994, San Jose, CA, United States

Abstract

In the emerging field of biotechnology, new technologies that enable early detection of genetic aberration or cancer are becoming a reality. These methods label specific parts of the DNA of the chromosomes with fluorescence fluors. The development of the techniques, as well as the correlation of the genetic aberrations to cancer, require a scientific imaging system coupled with an advanced image analysis method. In this paper we outline the problems associated with both the imaging and image analysis. We present a coherent approach to the design of the system. The system parameters are considered simultaneously so that the acquisition process is fast, yet the image quality is good so that its analysis is reliable. A fast new technique for scanning/focusing using extrapolation and interpolation is described. Moreover, a novel approach for a robust analysis through a combination of morphological operators and statistical optimization is outlined. Experiments and results that evaluate the system performance are presented.

Citation Download Citation

Amir Waks and Hershel Ginsburg "Multidisciplinary approach for detecting subcellular objects in low level light fluorescence microscopy", Proc. SPIE 2173, Image Acquisition and Scientific Imaging Systems, (5 May 1994); https://doi.org/10.1117/12.175171

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