High-resolution, multiple optical-mode confocal microscope: I. System design, image acquisition and 3D visualization

Carol J. Cogswell; Kieran G. Larkin; John W. O'Byrne; Matthew R. Arnison

doi:10.1117/12.172105

4 April 1994 High-resolution, multiple optical mode confocal microscope: I. System design, image acquisition and 3D visualization

Carol J. Cogswell, Kieran G. Larkin, John W. O'Byrne, Matthew R. Arnison

Author Affiliations +

Proceedings Volume 2184, Three-Dimensional Microscopy: Image Acquisition and Processing; (1994) https://doi.org/10.1117/12.172105
Event: IS&T/SPIE 1994 International Symposium on Electronic Imaging: Science and Technology, 1994, San Jose, CA, United States

Abstract

We have designed and constructed an experimental confocal specimen-scanning microscope which has the capability of producing high resolution 3D images in a variety of optical modes, many of which are not currently available on commercial confocal microscopes. The transmission Nomarski differential interference contrast mode is particularly interesting because it can be utilized to image small changes in refractive index within complex biological specimens which are transparent in standard brightfield. The three-color reflection configuration can produce a color 3D image, which means that stained or pigmented objects will be similar in appearance to images obtained from conventional white light microscopes which makes them more recognizable.

Citation Download Citation

Carol J. Cogswell, Kieran G. Larkin, John W. O'Byrne, and Matthew R. Arnison "High-resolution, multiple optical mode confocal microscope: I. System design, image acquisition and 3D visualization", Proc. SPIE 2184, Three-Dimensional Microscopy: Image Acquisition and Processing, (4 April 1994); https://doi.org/10.1117/12.172105

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available