Three-dimensional chemical imaging with a confocal scanning laser FT-Raman microscope

Colin J. H. Brenan; Ian Warwick Hunter

doi:10.1117/12.172080

4 April 1994 Three-dimensional chemical imaging with a confocal scanning laser Fourier transform-Raman microscope

Colin J. H. Brenan, Ian Warwick Hunter

Proceedings Volume 2184, Three-Dimensional Microscopy: Image Acquisition and Processing; (1994) https://doi.org/10.1117/12.172080
Event: IS&T/SPIE 1994 International Symposium on Electronic Imaging: Science and Technology, 1994, San Jose, CA, United States

Abstract

Traditional approaches in confocal microscopy have focussed on techniques that use elastically, or Rayleigh, scattered photons to generate volumetric intensity or phase images of an object. Common to these imaging modes is an inability to discriminate between optically similar but chemically distinct materials. We report in this paper on a new class of confocal microscope which uses inelastically, or Raman, scattered light to generate volumetric chemical images of a material. We designed and built a prototype instrument, called a confocal scanning laser FT-Raman microscope, which combines a confocal scanning laser microscope with a FT Raman spectrometer. The high depth and lateral spatial resolution of the confocal optics design defines a volume element from which the Raman scattered light is collected and then analyzed by the spectrometer for its spectral content. The sample is scanned through the microscope focal volume and a 3D chemical image is generated based on the content of the Raman spectrum measured at each scan position. The results to be presented include instrument characterization measurements and examples of volumetric chemical imaging.

Citation Download Citation

Colin J. H. Brenan and Ian Warwick Hunter "Three-dimensional chemical imaging with a confocal scanning laser Fourier transform-Raman microscope", Proc. SPIE 2184, Three-Dimensional Microscopy: Image Acquisition and Processing, (4 April 1994); https://doi.org/10.1117/12.172080

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