Laser-cytofluorescence microscopic image restoration by iterative deconvolution

Chengqi Xu; Eric Maire; Serge Jacquey

doi:10.1117/12.198723

4 January 1995 Laser-cytofluorescence microscopic image restoration by iterative deconvolution

Chengqi Xu, Eric Maire, Serge Jacquey

Proceedings Volume 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging; (1995) https://doi.org/10.1117/12.198723
Event: International Symposium on Biomedical Optics Europe '94, 1994, Lille, France

Abstract

The aim of our study is to improve the performances of an optical microscope by image deconvolution technique to attain that of a confocal one in the field of laser cytofluorescence. The fluorescence of antigen lymphocyte sites marked by rhodamine is induced by a laser ((lambda) equals 543 nm) or a mercury vapor lamp. A set of scanned fluorescence images is acquired at different focal planes by a SIT camera, fitted on an optical ZEISS microscope (N.A. 1.25, X100). The entire system is controlled by a PC equipped with a MATROX-MVP-AT image processing card. Since an optical microscope has a more important focal depth than a confocal one, an improved iterative deconvolution algorithm of Van Cittert's has been used to artificially reduce the focal depth. In order to ensure the convergence of such an iterative algorithm, a new criterion for relaxation coefficient is defined through a theoretical study. The experiments show an improvement of 50% in the spatial x-y resolution of 30% in optical z axis gain. As regards our application, this processing brings our system to a comparable performance level as a confocal microscope.

Citation Download Citation

Chengqi Xu, Eric Maire, and Serge Jacquey "Laser-cytofluorescence microscopic image restoration by iterative deconvolution", Proc. SPIE 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging, (4 January 1995); https://doi.org/10.1117/12.198723

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available