Automated laser spectrofluorimeter for biophysics and biomedicine

Alexander S. Kozlovski; Nicolai A. Nemkovich; Anatoly Nikolaevich Rubinov; Yurii V. Zvinevich

doi:10.1117/12.197492

2 January 1995 Automated laser spectrofluorimeter for biophysics and biomedicine

Alexander S. Kozlovski, Nicolai A. Nemkovich, Anatoly Nikolaevich Rubinov, Yurii V. Zvinevich

Proceedings Volume 2370, 5th International Conference on Laser Applications in Life Sciences; (1995) https://doi.org/10.1117/12.197492
Event: Laser Applications in Life Sciences: 5th International Conference, 1994, Minsk, Belarus

Abstract

We present automated laser spectrofluorimeter and some applications of it. Laser spectrofluorimeter is fully automated. The lasers and all optical and electronic devices are controlled by the computer. The laser spectrofluorimeter can be used for measuring stationary fluorescence and anisotropy spectra, time-resolved fluorescence characteristics with temporal resolution from subnanoseconds to milliseconds: (1) instantaneous florescence spectra (0.1 ns - 10 ms); (2) fluorescence kinetics and lifetime of the excited state (0.05ense - e10ems, after mathematical treatment); and (3) anisotropy spectra and anisotropy kinetics (0.1 ns - 10 ms). Experimental data can be treated with a full set of programs: (1) deconvolution of fluorescence pulse with up to 3-exponent approximation; (2) calculation of anisotropy kinetics with simultaneous deconvolution for different polarization; (3) calculation of instantaneous spectra from fluorescence decays; and (4) mathematical treatment of spectra (position of spectrum, halfwidth, asymmetry, etc). Laser spectrofluorimeter was tested on a number of objects (dye solutions, fluorescent probe in phospholipid bilayer and red blood cell membranes, humus substances in natural water, etc.) and demonstrated its high versatility.

Citation Download Citation

Alexander S. Kozlovski, Nicolai A. Nemkovich, Anatoly Nikolaevich Rubinov, and Yurii V. Zvinevich "Automated laser spectrofluorimeter for biophysics and biomedicine", Proc. SPIE 2370, 5th International Conference on Laser Applications in Life Sciences, (2 January 1995); https://doi.org/10.1117/12.197492

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