Interaction of instrumental and process uncertainties in the overall precision of clinical diagnostics

Gerald E. Cohn

doi:10.1117/12.206018

3 April 1995 Interaction of instrumental and process uncertainties in the overall precision of clinical diagnostics

Gerald E. Cohn

Proceedings Volume 2386, Ultrasensitive Instrumentation for DNA Sequencing and Biochemical Diagnostics; (1995) https://doi.org/10.1117/12.206018
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

Clinical biochemical analysis requires the interaction of the signal from an assay reaction product with a sequence of optical, mechanical, electronic and computational subassemblies. Each of these components can introduce an uncertainty into the final precision of measurement. Contributions from uncertainties in the concentration of sample chromophore differ from standard models of detector response in absorbance measurements. A Beer's Law absorbance model predicts that relative errors in concentration decrease momotonically with increasing absorbance. The product of molar absorptivity, path length and chromophore concentration uncertainty characterizes this effect. Precision of concentration must be controlled according to the particular molar absorptivity and detector response of the instrument. Assay chemistry factors dominate at low absorbance values, making high precision difficult at low absorbance.

Citation Download Citation

Gerald E. Cohn "Interaction of instrumental and process uncertainties in the overall precision of clinical diagnostics", Proc. SPIE 2386, Ultrasensitive Instrumentation for DNA Sequencing and Biochemical Diagnostics, (3 April 1995); https://doi.org/10.1117/12.206018

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