Photodynamic therapy of human bladder carcinoma cells in vitro with liposomes as carrier for porphycene: 9-acetamido-2,7,12,17-tetra-n-propylporphycene (AamTPPn)

Ella Dumbravia Reich; Ruediger Bachor; Thomas Zeizel; Klaus Kleinschmidt; Richard E. Hautmann

doi:10.1117/12.230952

31 January 1996 Photodynamic therapy of human bladder carcinoma cells in vitro with liposomes as carrier for porphycene: 9-acetamido-2,7,12,17-tetra-n-propylporphycene (AamTPPn)

Ella Dumbravia Reich, Ruediger Bachor, Thomas Zeizel, Klaus Kleinschmidt, Richard E. Hautmann

Author Affiliations +

Proceedings Volume 2625, Photochemotherapy: Photodynamic Therapy and Other Modalities; (1996) https://doi.org/10.1117/12.230952
Event: BiOS Europe '95, 1995, Barcelona, Spain

Abstract

In vitro experiments were performed on human bladder carcinoma cells (EJ, 28, Tumorbank Heidelberg, Germany) to evaluate the efficiency of photodynamic activity of AamTPPn encapsulated within liposomes. After irradiation the cells were incubated for 2 days at 37 degrees Celsius, then fixed, stained, counted and compared with the control group. By using a fluorescence microscope, we evaluated the intracellular uptake of AamTPPn in liposomes. After incubation of cells with liposomes-administered AamTPPn at a concentration of 40 (mu) g/ml dark toxicity occurred. In all experiments a drug and light dose dependent decrease in cell survival was found. By transmission electron microscopy marked changes were observed at the mitochondria with dissolution of the cristae and development of vacuols.

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Ella Dumbravia Reich, Ruediger Bachor, Thomas Zeizel, Klaus Kleinschmidt, and Richard E. Hautmann "Photodynamic therapy of human bladder carcinoma cells in vitro with liposomes as carrier for porphycene: 9-acetamido-2,7,12,17-tetra-n-propylporphycene (AamTPPn)", Proc. SPIE 2625, Photochemotherapy: Photodynamic Therapy and Other Modalities, (31 January 1996); https://doi.org/10.1117/12.230952

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