Laser trapping and phase conjugate microscopy for micromanipulation and image processing

Gregory J. Sonek; Wen Wang; Arthur E. T. Chiou; Michael W. Berns

doi:10.1117/12.239498

10 May 1996 Laser trapping and phase conjugate microscopy for micromanipulation and image processing

Gregory J. Sonek, Wen Wang, Arthur E. T. Chiou, Michael W. Berns

Proceedings Volume 2678, Optical Diagnostics of Living Cells and Biofluids; (1996) https://doi.org/10.1117/12.239498
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

We report the implementation of an optical laser trap that incorporates phase conjugate microscopy as one of its key elements. The laser trap consists of a primary trapping beam, and a counterpropagating self-pumped phase conjugate beam, the latter being derived from the primary signalbeam when it is transmitted through the sample and phase conjugated in a photorefractive crystal. Using a fundamental TEM₀₀ gaussian beam from an Ar-ion laser (514 nm), and a barium titanate (BaTiO₃) crystal in the CAT (total internal reflection) geometry, an optical laser trap was created using approximately 25 mW of laser power. Strong transverse optical confinement is reported for approximately 2.5 micrometers diameter polystyrene microspheres, using both high and low numerical aperture (e.g. 60 X, 0.85 N.A. and 10 X, 0.25 N.A.) objective lenses. By virtue of the phase conjugation process, the present geometry achieves self-alignment of the two counter-propagating beams, as well as photorefractive gain in the ratio of nearly 4:1. The system has the potential for implementing several novel image processing functions unique to the nonlinear phase conjugation process, on living cells confined to an optical laser trap, including aberration correction, contrast reversal, and novelty filtering, respectively.

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Gregory J. Sonek, Wen Wang, Arthur E. T. Chiou, and Michael W. Berns "Laser trapping and phase conjugate microscopy for micromanipulation and image processing", Proc. SPIE 2678, Optical Diagnostics of Living Cells and Biofluids, (10 May 1996); https://doi.org/10.1117/12.239498

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KEYWORDS

Phase conjugation

Image processing

Laser optics

Optical tweezers

Optical proximity correction

Microscopy

Crystals

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