Optical flow cytometry utilizing microfabricated silicon flow channels

Eric H. Altendorf; Elizabeth Iverson; David Schutte; Bernhard H. Weigl; Thor D. Osborn; Roya Sabeti; Paul Yager

doi:10.1117/12.239515

10 May 1996 Optical flow cytometry utilizing microfabricated silicon flow channels

Eric H. Altendorf, Elizabeth Iverson, David Schutte, Bernhard H. Weigl, Thor D. Osborn, Roya Sabeti, Paul Yager

Proceedings Volume 2678, Optical Diagnostics of Living Cells and Biofluids; (1996) https://doi.org/10.1117/12.239515
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

A benchtop optical flow cytometer utilizing microfabricated silicon V-groove flow channels (25 micron diameter) has been constructed and tested using diluted whole blood. A 1.2 mw diode laser probe beam focused onto the flow stream is used to generate scattered light from passing blood cells. Photodiode detectors are used to collect both small and large angle signals which are counted and analyzed for pulse peak intensities. Count rates as high as 1000/second have been obtained using pressure heads of about 0.5 psi. Optical modeling has also been carried out in order to determine the light scattering signature of blood cells passing down various channel flow lines. Results suggest that a significant amount of experimental signal variability may be due to variations in the positions of cells passing through the sampling region, but that this degree of signal variation should not prohibit the discrimination of different cell populations. Experimental and computational results are presented and discussed, as well as the possibility of developing a miniature portable flow cytometer based on microfabricated flow channels.

Citation Download Citation

Eric H. Altendorf, Elizabeth Iverson, David Schutte, Bernhard H. Weigl, Thor D. Osborn, Roya Sabeti, and Paul Yager "Optical flow cytometry utilizing microfabricated silicon flow channels", Proc. SPIE 2678, Optical Diagnostics of Living Cells and Biofluids, (10 May 1996); https://doi.org/10.1117/12.239515

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