Design of separation media for DNA sequencing in capillaries

Steve Menchen; Ben A. Johnson; Ramki Madabhushi; Mitchell A. Winnik

doi:10.1117/12.237618

1 April 1996 Design of separation media for DNA sequencing in capillaries

Steve Menchen, Ben A. Johnson, Ramki Madabhushi, Mitchell A. Winnik

Proceedings Volume 2680, Ultrasensitive Biochemical Diagnostics; (1996) https://doi.org/10.1117/12.237618
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

Practical DNA sequencing in a capillary format requires sieving fluids that can be replaced after each sequencing experiment. Uncrosslinked water soluble polymers were used to prepare DNA sieving networks, both through simple entanglements of linear polymers, and by end- capping with hydrophobic tails; aqueous solutions of the end-capped polymers yield viscous networks held together by micellar crosslinks. Both networks are effective resolving media for DNA sequencing ladders, and flow under stress, which has enabled the design of an automated capillary DNA sequencing system composed of a gel pump interfaced to an electrophoresis apparatus. The sieving properties of these polymers are described.

Citation Download Citation

Steve Menchen, Ben A. Johnson, Ramki Madabhushi, and Mitchell A. Winnik "Design of separation media for DNA sequencing in capillaries", Proc. SPIE 2680, Ultrasensitive Biochemical Diagnostics, (1 April 1996); https://doi.org/10.1117/12.237618

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