Development of a bacteriophage displayed peptide library and biosensor

Robert C. Chin; Noe Salazar; Michael W. Mayo; Victor Ivan Villavicencio; Richard B. Taylor; James P. Chambers; James J. Valdes

doi:10.1117/12.237607

1 April 1996 Development of a bacteriophage displayed peptide library and biosensor

Robert C. Chin, Noe Salazar, Michael W. Mayo, Victor Ivan Villavicencio, Richard B. Taylor, James P. Chambers, James J. Valdes

Author Affiliations +

Proceedings Volume 2680, Ultrasensitive Biochemical Diagnostics; (1996) https://doi.org/10.1117/12.237607
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

A miniaturized, handheld biosensor for identification of hazardous biowarfare agents with high specificity is being developed. An innovative biological recognition system based on bacteriophage displayed peptide receptors will be utilized in conjunction with the miniature biosensor technology being developed. A bacteriophage library has been constructed to provide the artificial receptors. The library can contain millions of bacteriophage with randomly displayed peptide sequences in the phage outer protein coat which act as binding sites for the agents of interest. This library will be used to 'bio-pan' for phages that bind to a number of toxins and infectious agents and can, thus, provide an endless supply of low cost, reliable, specific, and stable artificial receptors. The biosensor instrument will utilize evanescent wave, planar waveguide, far-red dyes, diode laser and miniature circuit technologies for performance and portability.

Citation Download Citation

Robert C. Chin, Noe Salazar, Michael W. Mayo, Victor Ivan Villavicencio, Richard B. Taylor, James P. Chambers, and James J. Valdes "Development of a bacteriophage displayed peptide library and biosensor", Proc. SPIE 2680, Ultrasensitive Biochemical Diagnostics, (1 April 1996); https://doi.org/10.1117/12.237607

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