High-speed electronics for the detection of time-resolved fluorescence in a continuous flow system

Rainer Erdmann; Uwe Ortmann; Joerg Enderlein; Wolfgang Becker; Michael Wahl; Edgar O. Klose

doi:10.1117/12.237635

1 April 1996 High-speed electronics for the detection of time-resolved fluorescence in a continuous flow system

Rainer Erdmann, Uwe Ortmann, Joerg Enderlein, Wolfgang Becker, Michael Wahl, Edgar O. Klose

Proceedings Volume 2680, Ultrasensitive Biochemical Diagnostics; (1996) https://doi.org/10.1117/12.237635
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

In single molecule detection by laser induced fluorescence, a main problem is the low signal to noise ratio due to scattering of the exciting laser light. One common approach to solve this problem is the application of time resolved techniques. Here we present a high speed electronic (based on a pair of PC cards) specially suited for detection of TCSPC curves in a continuous flow system. The whole system works on two different time scales: a millisecond time scale (every millisecond a complete TCSPC curve is measured and stored) and a picosecond time scale (showing the fluorescence decay). The technique present here is of particular interest for applications such as fast DNA sequencing, where a distinction between the different bases solely by the decay times of the attached fluorescence labels is conceivable.

Citation Download Citation

Rainer Erdmann, Uwe Ortmann, Joerg Enderlein, Wolfgang Becker, Michael Wahl, and Edgar O. Klose "High-speed electronics for the detection of time-resolved fluorescence in a continuous flow system", Proc. SPIE 2680, Ultrasensitive Biochemical Diagnostics, (1 April 1996); https://doi.org/10.1117/12.237635

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