Time-resolved excimer fluorescence spectroscopy in lipid membranes

Kwan Hon Cheng; Lucy Ruymgaart; Digala M. Kulawansa; Pentti Somerharju

doi:10.1117/12.236182

25 March 1996 Time-resolved excimer fluorescence spectroscopy in lipid membranes

Kwan Hon Cheng, Lucy Ruymgaart, Digala M. Kulawansa, Pentti Somerharju

Proceedings Volume 2705, Fluorescence Detection IV; (1996) https://doi.org/10.1117/12.236182
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

Free or conjugated pyrene molecules exhibit concentration dependent excimer fluorescence property. In membrane biophysics, single chain pyrene labeled fluorophores have been extensively used to investigate various physical properties of membranes, e.g., fusion, lateral distribution, stability, polymorphic phase behavior and lipid/protein interactions. Recently, intramolecular excimer forming dual-chain labeled dipyrenyl lipids have been proposed to probe the local conformation and dynamics of membranes. By systematically varying the length of pyrenyl chains of the probes, new information pertaining to the transverse distributions of nanosecond-resolved molecular dynamics of conjugated pyrene molecules can be revealed with spatial resolution approaching 2 - 4 angstrom along the direction perpendicular to the lipid/water interface. In this study, a 3-state kinetic model and a novel nonlinear least squares global analysis method have been used to recover the intralipid free volume and rotational and translational diffusion constants of conjugated pyrenes from the monomer and excimer fluorescence decays and steady state fluorescence intensity data. The transverse distributions of those intramolecular dynamics parameters in membranes with and without local curvatures are presented.

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Kwan Hon Cheng, Lucy Ruymgaart, Digala M. Kulawansa, and Pentti Somerharju "Time-resolved excimer fluorescence spectroscopy in lipid membranes", Proc. SPIE 2705, Fluorescence Detection IV, (25 March 1996); https://doi.org/10.1117/12.236182

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KEYWORDS

Luminescence

Molecules

Excimers

Fluorescence spectroscopy

Lanthanum

Head

Liquid crystals

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