Anion selective optodes: development of a fluorescent fiber optic sensor for the determination of nitrite activity

Susan L. R. Barker; Michael R. Shortreed; Raoul Kopelman

doi:10.1117/12.260605

10 December 1996 Anion selective optodes: development of a fluorescent fiber optic sensor for the determination of nitrite activity

Susan L. R. Barker, Michael R. Shortreed, Raoul Kopelman

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Proceedings Volume 2836, Chemical, Biochemical, and Environmental Fiber Sensors VIII; (1996) https://doi.org/10.1117/12.260605
Event: SPIE's 1996 International Symposium on Optical Science, Engineering, and Instrumentation, 1996, Denver, CO, United States

Abstract

The response of state of the art anion optodes often cannot be described in a thermodynamically exact manner because the ionic strength within the membrane phase of such optodes changes during the course of a titration. Incorporating lipophilic charge sites in the anion optode membranes provides a constant ionic strength in the membrane phase, the ability to measure anion activities, and a more thermodynamically describable system. This configuration has been used to create a micrometer-sized nitrite-selective optode. Recent elucidation of the many biological roles of nitric oxide (NO) has spurred interest in sensitive and selective detection of this molecule. In biological systems NO is converted to NO₂^- within 30 sec and the biological concentration of NO₂^- is normally on the micromolar level. The optode we have prepared contains a selective vitamin B₁₂ derivative ionophore, a fluorescent chromoionophore (ETH 2439 or ETH 5350), and lipophilic charge sites. These components are entrapped in a highly plasticized PVC matrix which is placed on the distal end of the fiber. Sensor characteristics such as limit of detection and reversibility are presented.

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Susan L. R. Barker, Michael R. Shortreed, and Raoul Kopelman "Anion selective optodes: development of a fluorescent fiber optic sensor for the determination of nitrite activity", Proc. SPIE 2836, Chemical, Biochemical, and Environmental Fiber Sensors VIII, (10 December 1996); https://doi.org/10.1117/12.260605

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