Combined treatment of photodynamic therapy and the topoisomerase I inhibitor camptothecin in growing V79 and NHIK 3025 cells

Jean-Michel Gaullier; Siv Kjersti Rodal; Johan Moan; Kristian Berg

doi:10.1117/12.260775

4 December 1996 Combined treatment of photodynamic therapy and the topoisomerase I inhibitor camptothecin in growing V79 and NHIK 3025 cells

Jean-Michel Gaullier, Siv Kjersti Rodal, Johan Moan, Kristian Berg

Author Affiliations +

Proceedings Volume 2924, Photochemotherapy: Photodynamic Therapy and Other Modalities II; (1996) https://doi.org/10.1117/12.260775
Event: BiOS Europe '96, 1996, Vienna, Austria

Abstract

The topoisomerase I (Topo I) inhibitor camptothecin (CPT) has been combined with photodynamic treatment (PDT) in V79 and NHIK 3025 cells. Meso-tetra (N-methyl-4-pyridyl) porphine (TMPyPH₂) was used as a photosensitizer. The dye has been shown to localize in granules in the cytoplasm of both cell lines. Some of the granularly located TMPyPH₂ is relocated to the cytosol after exposure to a light dose inactivation 70% of the cells. The human NHIK 3025 carcinoma from cervix were more resistant to PDT (D₅₀ approximately equals 0.33 J/cm²) and CPT (D₅₀ approximately equals 320 nM) than Chinese hamster V79 lung fibroblasts (PDT, D₂₀ approximately equals 0.3 J/cm² and CPT, D₂₀ approximately equals 55 nM). When the cells were treated with CPT for 18 hours before PDT, the combination of treatments led to slight synergistic effects for low CPT concentrations (up to 100 nM in NHIK 3025 cells) and high PDT doses (above 0.15 J/cm²). For higher CPT concentrations and lower PDT doses, the combination of treatment became additive.

Citation Download Citation

Jean-Michel Gaullier, Siv Kjersti Rodal, Johan Moan, and Kristian Berg "Combined treatment of photodynamic therapy and the topoisomerase I inhibitor camptothecin in growing V79 and NHIK 3025 cells", Proc. SPIE 2924, Photochemotherapy: Photodynamic Therapy and Other Modalities II, (4 December 1996); https://doi.org/10.1117/12.260775

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