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The aim of the study was to determine the dose-dependent influence of low-power laser radiation on carbohydrate metabolism in 70 male Wistar rats. The animals were primarily divided into 2 groups: B - irradiated group and C - control one in which sham - irradiation was made. The rats from B - group were irradiated daily for 10 minutes with semiconductive laser emitting the radiation of infrared wavelength 904 nm. Within both groups the animals were divided into subgroups (B I - B VII and CI - C VII) in which the dissections were made on 1st, 3rd, 6th, 9th, and 14th day of irradiation and on 5th and 8th day after the end of cycle of irradiation respectively. In all subgroups blood samples were collected to determine the glucose and insulin levels. Parts of the liver and pancreas were taken for histological examination in light microscope and in electron microscope. The lowest, statistically significant glycaemia was observed in the subgroup B V. Significant increase of glycaemia and significantly higher insulin concentration was found only in the subgroup B VI. The I/G ratio increased significantly in the subgroup B V. Lower intensity of paS reaction was presented in subgroups B I, B III, B V, B VI and B VII. The increased amount of paS-positive substances was observed in the I and II zone of liver acinus. Electron microscopic studies of hepatocytes showed: numerous glycogen conglomerations in subgroups B I, B II, B VI and B VII, the extension of RER in B II and B III, light vacuoles in B II, Golgi apparatus and biliary canaliculus expansion in B V and structural changes of several mitochondria - slight swelling or discontinuation of their outer membranes, electron microscopic findings in pancreas cells included: lower number of typical granules in beta and alpha cells as well as Golgi apparatus results it was concluded that the influence of low power laser radiation on carbohydrate metabolism in generally insignificant. It is observed only for higher doses of radiation and does not cause persistent negative effects.
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