Pump-during-probe fluorometry of phytoplankton: group-specific photosynthetic characteristics from individual cell analysis

Alexander M. Chekalyuk; Robert J. Olson; H.M. Sosik

doi:10.1117/12.266411

6 February 1997 Pump-during-probe fluorometry of phytoplankton: group-specific photosynthetic characteristics from individual cell analysis

Alexander M. Chekalyuk, Robert J. Olson, H.M. Sosik

Author Affiliations +

Proceedings Volume 2963, Ocean Optics XIII; (1997) https://doi.org/10.1117/12.266411
Event: Ocean Optics XIII, 1996, Halifax, Nova Scotia, Canada

Abstract

Saturating-flash fluorescence techniques are used to monitor the state of the photosynthetic apparatus in phytoplankton under natural conditions. At present these are bulk water measurements, which produce estimates of average properties of all the fluorescent particles present in a sample. Here we describe an improved approach for single-cell measurements of phytoplankton. We have combined individual- cell 'pump-during-probe' measurements of chlorophyll fluorescence induction on the time scale of 30 to 100 microseconds with flow cytometric characterization of each cell, to obtain population-specific photosynthetic characteristics. The results provide information about the potential quantum yield of photochemistry, the fraction of functional reaction centers, and the functional absorption cross section for photosystem 2.

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Alexander M. Chekalyuk, Robert J. Olson, and H.M. Sosik "Pump-during-probe fluorometry of phytoplankton: group-specific photosynthetic characteristics from individual cell analysis", Proc. SPIE 2963, Ocean Optics XIII, (6 February 1997); https://doi.org/10.1117/12.266411

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