Comparison of experiment and theory for finite-sized multiple-aperture arrays in direct-view microscopy

Eithne M. McCabe; D. T. Fewer; Simon J. Hewlett

doi:10.1117/12.271255

10 April 1997 Comparison of experiment and theory for finite-sized multiple-aperture arrays in direct-view microscopy

Eithne M. McCabe, D. T. Fewer, Simon J. Hewlett

Proceedings Volume 2984, Three-Dimensional Microscopy: Image Acquisition and Processing IV; (1997) https://doi.org/10.1117/12.271255
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

Experimental results are given for the optical sectioning characteristics of finite-sized, multiple-aperture arrays in brightfield direct-view microscopy. We also present a theoretical model for the brightfield direct-view microscope (DVM). This allows us to determine the optical sectioning strength for finite-sized, multiple-pinhole arrays with an arbitrary distribution of apertures. The theoretical model is modified, for experimental purposes, to take account of the presence of objective lens pupil shading. A comparison between experimental and theoretical results for the axial response of the DVM to a plane mirror specimen is presented. In particular, the effects of pinhole size, pinhole spacing and array geometry are investigated in detail with a veil to (1) achieving good optical sectioning characteristics and (2) maximizing the amount of light available for imaging. The implications of our results for practical systems as regards pinhole-array design and fabrication are also discussed.

Citation Download Citation

Eithne M. McCabe, D. T. Fewer, and Simon J. Hewlett "Comparison of experiment and theory for finite-sized multiple-aperture arrays in direct-view microscopy", Proc. SPIE 2984, Three-Dimensional Microscopy: Image Acquisition and Processing IV, (10 April 1997); https://doi.org/10.1117/12.271255

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available