Simple method of enzyme immobilization for pH-ISFET-based urea biosensors

Dorota Pijanowska; Wladislaw Torbicz

doi:10.1117/12.266713

13 February 1997 Simple method of enzyme immobilization for pH-ISFET-based urea biosensors

Dorota Pijanowska, Wladislaw Torbicz

Proceedings Volume 3054, Optoelectronic and Electronic Sensors II; (1997) https://doi.org/10.1117/12.266713
Event: Optoelectronic and Electronic Sensors II, 1996, Szczyrk, Poland

Abstract

In this paper, a simple chemical method of urease immobilization on silicon nitride surface is described. As a basic structure to construct urea-biosensor, a pH-sensitive Si₃N₄-gate ISFET was used. The developed method of chemical immobilization of urease is based on Schiff's base formation. The developed EnFET type urea biosensor are characterized by the following parameters: (1) maximum analytical signal: 120 divided by 140 mV in 10 mM phosphate buffer solution, (2) linear range of the (Delta) U_gs equals f(logC_urea): pC_urea(2 divided by 3.5) in 10 mM phosphate buffer, (3) response time: 80 s and (4) lifetime: 35 days with the stable analytical signal then after 52 days this signal decreased by at least 40%. The influence of the concentration as well as the pH of the buffer solution on EnFET response were investigated.

Citation Download Citation

Dorota Pijanowska and Wladislaw Torbicz "Simple method of enzyme immobilization for pH-ISFET-based urea biosensors", Proc. SPIE 3054, Optoelectronic and Electronic Sensors II, (13 February 1997); https://doi.org/10.1117/12.266713

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