Temperature control of a microspectrophotometer system for monitoring the redox reactions of respiratory pigments in small volumes

Karen Y. Kavanagh; James Edward Walsh; J. Murphy; M. Harmey; M. A. Farrell; O. Hardimann; R. Perryman

doi:10.1117/12.308038

1 May 1998 Temperature control of a microspectrophotometer system for monitoring the redox reactions of respiratory pigments in small volumes

Karen Y. Kavanagh, James Edward Walsh, J. Murphy, M. Harmey, M. A. Farrell, O. Hardimann, R. Perryman

Author Affiliations +

Proceedings Volume 3253, Biomedical Sensing and Imaging Technologies; (1998) https://doi.org/10.1117/12.308038
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

We report the development of a microspectrophotometer system for use on micro samples of mitochondrial respiratory pigments. A novel optical fiber set-up uses visible spectrophotometry to monitor the reduction of mitochondrial electron carriers. Data is presented for the reduction of cytochrome-c and for the effect of temperature on the levels of complex II/III activity from the mitochondria of rat liver. This in-vivo simulation of the reduction of cytochrome-c can be observed using a fiber optic probe which requires less than twenty (mu) l of sample for analysis. The key features of the system are: front end adaptability, high sensitivity and fast multispectral acquisition which are essential for the biological reactions which are observed.

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Karen Y. Kavanagh, James Edward Walsh, J. Murphy, M. Harmey, M. A. Farrell, O. Hardimann, and R. Perryman "Temperature control of a microspectrophotometer system for monitoring the redox reactions of respiratory pigments in small volumes", Proc. SPIE 3253, Biomedical Sensing and Imaging Technologies, (1 May 1998); https://doi.org/10.1117/12.308038

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