Clinical infrared spectroscopy: multiple antigen determination by solid-phase infrared immunoassay and homogeneous infrared immunoassay

Ashraf A. Ismail; Steven M. Barnett

doi:10.1117/12.306075

24 April 1998 Clinical infrared spectroscopy: multiple antigen determination by solid-phase infrared immunoassay and homogeneous infrared immunoassay

Ashraf A. Ismail, Steven M. Barnett

Author Affiliations +

Proceedings Volume 3257, Infrared Spectroscopy: New Tool in Medicine; (1998) https://doi.org/10.1117/12.306075
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

In IR immunoassay, organometallic markers containing metal- carbonyl moieties are employed in conjunction with FTIR spectroscopy as a detection method. These metal-carbonyl markers have characteristic and intense absorption bands in a region of the IR spectrum that is devoid of other strong absorptions and can be detected at pixomole levels. In addition, the ability to individually detect different metal-carbonyl markers in the presence of each other affords the possibility of determining multiple antigens in a single test. In the present work, we have undertaken the simplification and automation of the IR immunoassay method in order to increase its potential utility for routine use. A solid-phase IR immunoassay test has been developed in which antibodies specific to the analytes of interest are adsorbed on the surface of an IR-transparent membrane. In an alternative homogenous immunoassay format, antigens are adsorbed on the surface of an internal reflection element (IRE), and the binding of these antigens to the corresponding antibodies in a solution placed in contact with the IRE is monitored by attenuated total reflectance spectroscopy. Competition between the adsorbed antigens and free antigens in the solution for the antibody binding sites then provides the basis for a homogenous immunoassay.

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Ashraf A. Ismail and Steven M. Barnett "Clinical infrared spectroscopy: multiple antigen determination by solid-phase infrared immunoassay and homogeneous infrared immunoassay", Proc. SPIE 3257, Infrared Spectroscopy: New Tool in Medicine, (24 April 1998); https://doi.org/10.1117/12.306075

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