Advances in photon counting for bioluminescence

Martin B. Ingle; J. Ralph Powell

doi:10.1117/12.331221

18 November 1998 Advances in photon counting for bioluminescence

Martin B. Ingle, J. Ralph Powell

Proceedings Volume 3434, Image Intensifiers and Applications; and Characteristics and Consequences of Space Debris and Near-Earth Objects; (1998) https://doi.org/10.1117/12.331221
Event: SPIE's International Symposium on Optical Science, Engineering, and Instrumentation, 1998, San Diego, CA, United States

Abstract

Photon counting systems were originally developed for astronomy, initially by the astronomical community. However, a major application area is in the study of luminescent probes in living plants, fishes and cell cultures. For these applications, it has been necessary to develop camera system capability at very low light levels -- a few photons occasionally -- and also at reasonably high light levels to enable the systems to be focused and to collect quality images of the object under study. The paper presents new data on MTF at extremely low photon flux and conventional ICCD illumination, counting efficiency and dark noise as a function of temperature.

Citation Download Citation

Martin B. Ingle and J. Ralph Powell "Advances in photon counting for bioluminescence", Proc. SPIE 3434, Image Intensifiers and Applications; and Characteristics and Consequences of Space Debris and Near-Earth Objects, (18 November 1998); https://doi.org/10.1117/12.331221

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