High-frequency demodulation of multiphoton fluorescence in hyper-Raleigh scattering

Geert Olbrechts; Tom Munters; Koen J. Clays; Andre P. Persoons

doi:10.1117/12.328566

29 October 1998 High-frequency demodulation of multiphoton fluorescence in hyper-Raleigh scattering

Geert Olbrechts, Tom Munters, Koen J. Clays, Andre P. Persoons

Proceedings Volume 3474, Second-Order Organic Nonlinear Optics; (1998) https://doi.org/10.1117/12.328566
Event: SPIE's International Symposium on Optical Science, Engineering, and Instrumentation, 1998, San Diego, CA, United States

Abstract

Femtosecond hyper-Rayleigh scattering (HRS) is used for the suppression of multi-photon fluorescence contributions to the apparent HRS signal. The intrinsic high harmonic content of the femtosecond pulse is used as a high frequency amplitude modulation source. Due to the nonzero fluorescence lifetime, a high amplitude modulation frequency will result in an amplitude demodulated and phase shifted fluorescence signal. At very high modulation frequencies, the fluorescence signal becomes completely demodulated and only the inherent HRS signal will remain. Experimental verification has been obtained by the fluorescence suppression for a well known centrosymmetric fluorophore, 9,10-diphenylanthracene, added to a solution of a well characterized nonlinear optical chromophore, crystal violet. A comparison has also been made between the first hyperpolarizability value (beta) of a nonfluorescent ionic hemicyanine dye and the (beta) value of the fluorescent inclusion complex of the dye incorporated in an amylose matrix. The inherent fluorescence-free (beta) value for the complex appeared to be twice the value for the dye itself.

Citation Download Citation

Geert Olbrechts, Tom Munters, Koen J. Clays, and Andre P. Persoons "High-frequency demodulation of multiphoton fluorescence in hyper-Raleigh scattering", Proc. SPIE 3474, Second-Order Organic Nonlinear Optics, (29 October 1998); https://doi.org/10.1117/12.328566

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