Frequency-domain pump-probe microscopic imaging using intensity-modulated laser diodes

Christof Buehler; Chen-Yuan Dong; Peter T. C. So; Todd E. French; Enrico Gratton

doi:10.1117/12.346749

21 April 1999 Frequency-domain pump-probe microscopic imaging using intensity-modulated laser diodes

Christof Buehler, Chen-Yuan Dong, Peter T. C. So, Todd E. French, Enrico Gratton

Proceedings Volume 3603, Systems and Technologies for Clinical Diagnostics and Drug Discovery II; (1999) https://doi.org/10.1117/12.346749
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

We report the implementation of intensity modulated diode lasers in frequency-domain pump-probe studies, diode lasers are compact, stable, and economical units that require little maintenance. In our study, a 365 nm diode laser is used as the excitation source and the output of a 680 nm unit induces stimulated emission from excited state fluorophores. By modulating the intensities of the two diode lasers at slightly different frequencies, and detecting the fluorescence signal at the cross-correlation frequency, both time-resolved and high spatial resolution imaging can be achieved. The laser diodes are modulated in the 100 MHz cross-correlation signal has been used for time-resolved imaging of fluorescent microspheres and mouse fibroblasts labeled with nucleic acid stains TOTO-3. These results demonstrate and feasibility of using intensity modulated diode lasers for frequency-domain, pump-probe studies.

Citation Download Citation

Christof Buehler, Chen-Yuan Dong, Peter T. C. So, Todd E. French, and Enrico Gratton "Frequency-domain pump-probe microscopic imaging using intensity-modulated laser diodes", Proc. SPIE 3603, Systems and Technologies for Clinical Diagnostics and Drug Discovery II, (21 April 1999); https://doi.org/10.1117/12.346749

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