Microvolume laser scanning cytometry platform for biological marker discovery

Ian D. Walton; Louis J. Dietz; Gary Frenzel; Jerry Chen; Jim Winkler; Scott M. Norton; Aaron Kantor

doi:10.1117/12.380512

22 March 2000 Microvolume laser scanning cytometry platform for biological marker discovery

Ian D. Walton, Louis J. Dietz, Gary Frenzel, Jerry Chen, Jim Winkler, Scott M. Norton, Aaron Kantor

Proceedings Volume 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing; (2000) https://doi.org/10.1117/12.380512
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

Modern chemical synthesis and screening technologies have the ability to create large numbers of lead components but still do not answer questions of efficacy, dosing, toxicity and optimal patient population. SurroMed was founded to develop discovery technologies for new biological markers that will answer these questions. Biological markers will be derived from the results of many different assays; cell surface, serum factors and others, many performed using whole blood and other fluids and tissues. We report on the design of a Microvolume Laser Scanning Cytometer (MLSC) and disposable capillary arrays to be used in biological marker discovery. The MLSC machines are used primarily for cell surface assays, though they are suitable for other fluorescence assays as well. Each capillary requires a very small sample volume per assay, less than twenty micro- liters, and so allows hundreds of assays to be performed on a single ten milliliter blood draw. The new MLSC is capable of optimally detecting four fluorescence colors at different scan rates. HeNe excitation and red emission permits the use of whole blood, so that no lysing or cell separation is required. The MLSC instrument and disposable capillary arrays are in routine use for biological marker discovery at SurroMed.

Citation Download Citation

Ian D. Walton, Louis J. Dietz, Gary Frenzel, Jerry Chen, Jim Winkler, Scott M. Norton, and Aaron Kantor "Microvolume laser scanning cytometry platform for biological marker discovery", Proc. SPIE 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing, (22 March 2000); https://doi.org/10.1117/12.380512

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