Paper
14 April 2000 Confocal imaging of biological tissues using second harmonic generation
Author Affiliations +
Abstract
A confocal microscopy imaging system was devised to selectively detect second harmonic signals generated by biological tissues. Several types of biological tissues were examined using this imaging system, including human teeth, bovine blood vessels, and chicken skin. All these tissues generated strong second harmonic signals. There is considerable evidence that the source of these signals in tissue is collagen. Collagen, the predominant component of most tissues, is known to have second order nonlinear susceptibility. This technique may have diagnostic usefulness in pathophysiological conditions characterized by changes in collagen structure including malignant transformation of nevi, progression of diabetic complications, and abnormalities in wound healing.
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Beop-Min Kim, Patrick Christian Stoller, Karen M. Reiser, Juergen P. Eichler, Ming Yan, Alexander M. Rubenchik, and Luiz Barroca Da Silva "Confocal imaging of biological tissues using second harmonic generation", Proc. SPIE 3927, Optical Pulse and Beam Propagation II, (14 April 2000); https://doi.org/10.1117/12.382051
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KEYWORDS
Tissues

Collagen

Confocal microscopy

Second-harmonic generation

Signal detection

Skin

Harmonic generation

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